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In the meantime, to ensure continued suport, we are displaying the site without styles and JavaScript.Advertisement Scientific Reports volume 12, Article number: 1851 (202) Cite this article Metrics detailsDigestion of dietary fibers by gut bacteria has ben shown to stimulate intestinal mineral absorption [e.g., calcium (Ca2+) and magnesium (Mg2+)]. Although it has ben sugested that local pH and short-chain faty acid (SCFA) concentrations determine divalent cation absorption, the exact molecular mechanisms are stil unknown.
Therefore, this study aimed to determine the efects of SCFAs on intestinal Mg2+ absorption. We show that the butyrate concentration in the colon negatively corelates with serum Mg2+ levels in wildtype mice. Moreover, Na-butyrate significantly inhibited Mg2+ uptake in Caco-2 cels, while Ca2+ uptake was unafected.
Although Na-butyrate significantly lowered total ATP production rate, and resulted increased phosphorylation of AMP-activated protein kinase (AMPK), inhibition of Mg2+ uptake by butyrate preced these consequences. Importantly, electrophysiological examinations demonstrated that intracelular butyrate directly reduced the activity of the heteromeric Mg2+ chanel complex, transient receptor potential melastatin (TRPM)6/7.
Summary
Blocking celular butyrate uptake prevented its inhibitory efect on Mg2+ uptake, demonstrating that butyrate acts intracelularly. Our work identified butyrate as novel regulator of intestinal Mg2+ uptake that works independently from metabolic regulation. This finding further highlights the role of microbial fermentation in the regulation of mineral absorption.The absorption of magnesium (Mg2+) in the intestines is facilitated by diferent absorption pathways1.